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destain buffer  (Beyotime)


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    Structured Review

    Beyotime destain buffer
    Destain Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/destaining+buffer/destain+buffer/pmc11425246-294-14-16
    Average 90 stars, based on 1 article reviews
    destain buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Marker:

    Article Title: In vitro digestion and cellular antioxidant activity of β-carotene-loaded emulsion stabilized by soy protein isolate-Pleurotus eryngii polysaccharide conjugates
    Article Snippet: β-Carotene is a natural fat-soluble antioxidant, while the poor oral bioavailability of β-carotene restricts its application for functional foods.. To overcome this barrier, a stable oil-in-water emulsion was structured to effectively deliver β-carotene.. In this study, soy protein isolate (SPI)-Pleurotus eryngii polysaccharide (PEP) conjugate was prepared by Maillard reaction under controlled wet-heating conditions. β-Carotene was encapsulated in SPI-PEP conjugate-stabilized emulsion and its gastrointestinal behavior and antioxidant activity were assessed in vitro.

    Electrophoresis:

    Article Title: In vitro digestion and cellular antioxidant activity of β-carotene-loaded emulsion stabilized by soy protein isolate-Pleurotus eryngii polysaccharide conjugates
    Article Snippet: β-Carotene is a natural fat-soluble antioxidant, while the poor oral bioavailability of β-carotene restricts its application for functional foods.. To overcome this barrier, a stable oil-in-water emulsion was structured to effectively deliver β-carotene.. In this study, soy protein isolate (SPI)-Pleurotus eryngii polysaccharide (PEP) conjugate was prepared by Maillard reaction under controlled wet-heating conditions. β-Carotene was encapsulated in SPI-PEP conjugate-stabilized emulsion and its gastrointestinal behavior and antioxidant activity were assessed in vitro.

    Article Title: Co-Exposure to Formaldehyde and Acrolein Generates a New Protein Adduct Activating RAGE.
    Article Snippet: Reactive carbonyl species (RCS), sourced exogenously and endogenously, can modify proteins to generate advanced glycation end products (AGEs), which can lead to cell damage and various diseases.. To date, it has not been reported that two or more RCSs can modify a single amino acid residue in proteins.. The aim of the present study is to investigate whether and how formaldehyde and acrolein simultaneously modify lysine residues in proteins and whether the resulting adducts are capable of binding to the AGE receptor (RAGE).

    Article Title: Diffuse Large B-Cell Lymphoma Promotes Endothelial-to-Mesenchymal Transition via WNT10A/Beta-Catenin/Snail Signaling
    Article Snippet: .. Following electrophoresis, gels were washed in buffer containing 2.5% Triton-X 100, 50 mM of Tris-HCl, 5 mM of Cacl 2 , and 1 μM of Zncl 2 and incubated in buffer containing 50 mM of Tris-HCl, 150 mM of Nacl, 10 mM of Cacl 2 , 1 μM of Zncl 2 , and 0.02% Brij for 48 h. After being stained by Coomassie Blue Staining Solution (Beyotime, China) for 3 h, gels were washed in Coomassie Blue Staining Destaining Solution (Beyotime, China). .. Images were captured by Gel DocTM EZ Imager (Bio-Rad, Hercules, CA, USA).

    Article Title: Evidence for exposure biomarkers in dictamnine-induced liver injury resulting from metabolic activation in vitro and in mice.
    Article Snippet: Funding information This work was supported by the National Natural Science Foundation of China (grant no. 82174070).. Abstract Dictamnine (DTN), a furoquinoline alkaloid isolated from Dictamni Cortex, is responsible for the liver injury caused by Dictamni Cortex and the preparations.. Discovering new biomarkers with high specificity and sensitivity for diagnosis and tracing the source of DTN-induced liver injury is urgently needed.

    Imaging:

    Article Title: In vitro digestion and cellular antioxidant activity of β-carotene-loaded emulsion stabilized by soy protein isolate-Pleurotus eryngii polysaccharide conjugates
    Article Snippet: β-Carotene is a natural fat-soluble antioxidant, while the poor oral bioavailability of β-carotene restricts its application for functional foods.. To overcome this barrier, a stable oil-in-water emulsion was structured to effectively deliver β-carotene.. In this study, soy protein isolate (SPI)-Pleurotus eryngii polysaccharide (PEP) conjugate was prepared by Maillard reaction under controlled wet-heating conditions. β-Carotene was encapsulated in SPI-PEP conjugate-stabilized emulsion and its gastrointestinal behavior and antioxidant activity were assessed in vitro.

    Article Title: Co-Exposure to Formaldehyde and Acrolein Generates a New Protein Adduct Activating RAGE.
    Article Snippet: Reactive carbonyl species (RCS), sourced exogenously and endogenously, can modify proteins to generate advanced glycation end products (AGEs), which can lead to cell damage and various diseases.. To date, it has not been reported that two or more RCSs can modify a single amino acid residue in proteins.. The aim of the present study is to investigate whether and how formaldehyde and acrolein simultaneously modify lysine residues in proteins and whether the resulting adducts are capable of binding to the AGE receptor (RAGE).

    Staining:

    Article Title: In vitro digestion and cellular antioxidant activity of β-carotene-loaded emulsion stabilized by soy protein isolate-Pleurotus eryngii polysaccharide conjugates
    Article Snippet: β-Carotene is a natural fat-soluble antioxidant, while the poor oral bioavailability of β-carotene restricts its application for functional foods.. To overcome this barrier, a stable oil-in-water emulsion was structured to effectively deliver β-carotene.. In this study, soy protein isolate (SPI)-Pleurotus eryngii polysaccharide (PEP) conjugate was prepared by Maillard reaction under controlled wet-heating conditions. β-Carotene was encapsulated in SPI-PEP conjugate-stabilized emulsion and its gastrointestinal behavior and antioxidant activity were assessed in vitro.

    Article Title: Co-Exposure to Formaldehyde and Acrolein Generates a New Protein Adduct Activating RAGE.
    Article Snippet: Reactive carbonyl species (RCS), sourced exogenously and endogenously, can modify proteins to generate advanced glycation end products (AGEs), which can lead to cell damage and various diseases.. To date, it has not been reported that two or more RCSs can modify a single amino acid residue in proteins.. The aim of the present study is to investigate whether and how formaldehyde and acrolein simultaneously modify lysine residues in proteins and whether the resulting adducts are capable of binding to the AGE receptor (RAGE).

    Article Title: Allergenicity evaluation of fermented milk prepared by co-fermentation of Lactobacillus plantarum 7-2 and commercial starters after in vitro digestive.
    Article Snippet: The sample gels were stained with Coomassie Blue Staining Solution (Beyotime Biotechnology, Shanghai, China). .. The gels were then decolorized with Coomassie Blue Staining Destaining Solution (Beyotime Biotechnology, Shanghai, China). .. Azure C300 Gel Imaging System (Azure Biosystems, California, USA) is used for protein band imaging.

    Article Title: Natural borneol improves cellular uptake of curcumin to enhance its photodynamic bactericidal activity against Escherichia coli ATCC 8739.
    Article Snippet: Photodynamic inactivation (PDI), a non-thermal sterilization method, has attracted considerable attention due to its broad-spectrum antimicrobial activity, environmental friendliness and cost-effectiveness.. Curcumin (Cur), a food-grade photosensitizer, exhibits photodynamic antimicrobial activity based primarily on its efficiency in intracellular accumulation.. However, Cur’s low water solubility and the barriers presented by the outer membrane of Gram-negative bacteria challenge its ability to penetrate the cytoplasm.

    Article Title: Postsynaptic lncRNA Sera /Pkm2 pathway orchestrates the transition from social competition to rank by remodeling the neural ensemble in mPFC
    Article Snippet: IP sample and input control were boiled with SDS buffer for 10 min. After proteins were separated on 10% SDS–PAGE gels, gels were stained with Coomassie Brilliant Blue R250 (P0017B, Beyotime) for 2 h at room temperature. .. After washed with Coomassie Blue Staining Destaining Solution (P0017C, Beyotime) for 4 times overnight, protein bands were cut and pre-processed with acetonitrile. .. Samples were separated with UltiMate 3000 RSLCnano (Thermo Scientific), and the mass spectrometry was performed using Q Exactive Plus LCMS system (Thermo Scientific).

    Article Title: Diffuse Large B-Cell Lymphoma Promotes Endothelial-to-Mesenchymal Transition via WNT10A/Beta-Catenin/Snail Signaling
    Article Snippet: .. Following electrophoresis, gels were washed in buffer containing 2.5% Triton-X 100, 50 mM of Tris-HCl, 5 mM of Cacl 2 , and 1 μM of Zncl 2 and incubated in buffer containing 50 mM of Tris-HCl, 150 mM of Nacl, 10 mM of Cacl 2 , 1 μM of Zncl 2 , and 0.02% Brij for 48 h. After being stained by Coomassie Blue Staining Solution (Beyotime, China) for 3 h, gels were washed in Coomassie Blue Staining Destaining Solution (Beyotime, China). .. Images were captured by Gel DocTM EZ Imager (Bio-Rad, Hercules, CA, USA).

    Article Title: Evidence for exposure biomarkers in dictamnine-induced liver injury resulting from metabolic activation in vitro and in mice.
    Article Snippet: Funding information This work was supported by the National Natural Science Foundation of China (grant no. 82174070).. Abstract Dictamnine (DTN), a furoquinoline alkaloid isolated from Dictamni Cortex, is responsible for the liver injury caused by Dictamni Cortex and the preparations.. Discovering new biomarkers with high specificity and sensitivity for diagnosis and tracing the source of DTN-induced liver injury is urgently needed.

    Software:

    Article Title: Co-Exposure to Formaldehyde and Acrolein Generates a New Protein Adduct Activating RAGE.
    Article Snippet: Reactive carbonyl species (RCS), sourced exogenously and endogenously, can modify proteins to generate advanced glycation end products (AGEs), which can lead to cell damage and various diseases.. To date, it has not been reported that two or more RCSs can modify a single amino acid residue in proteins.. The aim of the present study is to investigate whether and how formaldehyde and acrolein simultaneously modify lysine residues in proteins and whether the resulting adducts are capable of binding to the AGE receptor (RAGE).

    Incubation:

    Article Title: Diffuse Large B-Cell Lymphoma Promotes Endothelial-to-Mesenchymal Transition via WNT10A/Beta-Catenin/Snail Signaling
    Article Snippet: .. Following electrophoresis, gels were washed in buffer containing 2.5% Triton-X 100, 50 mM of Tris-HCl, 5 mM of Cacl 2 , and 1 μM of Zncl 2 and incubated in buffer containing 50 mM of Tris-HCl, 150 mM of Nacl, 10 mM of Cacl 2 , 1 μM of Zncl 2 , and 0.02% Brij for 48 h. After being stained by Coomassie Blue Staining Solution (Beyotime, China) for 3 h, gels were washed in Coomassie Blue Staining Destaining Solution (Beyotime, China). .. Images were captured by Gel DocTM EZ Imager (Bio-Rad, Hercules, CA, USA).

    other:

    Article Title: Effects of deheading and rinsing pretreatment on the quality of white leg shrimp (Litopenaeus vannamei) surimi based on endogenous proteases.
    Article Snippet: In this study, the effects of different pretreatments on the quality of white leg shrimp surimi were investigated based on shrimp endogenous proteases.. The results showed that removing the head and rinsing significantly (P < 0.05) improved the gel strength, texture, whiteness, water distribution, and microstructure of the shrimp surimi gels.. Headless shrimp surimi (HSS) had higher salt-soluble protein and lower water-soluble protein than whole shrimp surimi (WSS).



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